Metabolism of Sinapine in Mustard Plants. II. Purification & Some Properties of Sinapine Esterase.
نویسنده
چکیده
Experimental evidence for the presence in mustard seedlings of an esterase that catalyzes the hydrolysis of sinapine into sinapic acid and choline has been presented previously (12). It was felt that further studies on this enzyme might be of some general biochemical interest. A preparation of sinapine esterase purified 20fold has been obtained from white mustard seedlings (Brassica hirta Moench). The specificity, pH optimum, and other properties of the enzyme have been examined and will be discussed in this paper. A spectrophotometric method for following the hydrolysis of sinapine based on the absorption of sinapic acid at 290 mn has been devised and is described.
منابع مشابه
Partial Purification and Some Properties of l-Sinapoylglucose: Choline Sinapoyltransferase (“Sinapine Synthase”) from Seeds of Raphanus sativus L. and Sinapis alba L
Hydroxycinnamoyltransferases which catalyze the formation of O-sinapoylcholine (sinapine) using l-0-sinapoyl-ß-D-glucose as acyl donor have been isolated from seeds of radish (Raphanus sativus L. var. sativus) and mustard (Sinapis alba L.) and purified 420and 293-fold, respectively. The enzymes (“sinapine synthase”) had apparent molecular weights of about 60,000 daltons and showed highest activ...
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ورودعنوان ژورنال:
- Plant physiology
دوره 38 2 شماره
صفحات -
تاریخ انتشار 1963